Archive for the ‘Fluorescence’ Category

Conformational Analysis of Lysozyme Using Intrinsic Tryptophan Fluorescence

Monday, June 6th, 2011

Introduction:
Tryptophan residues in a protein result in inherent fluorescence with UV excitation. As the protein goes from a folded (native) to denatured state, the emission spectrum will change slightly reflecting the change in folding state. (more…)

Fluorescence of Quinine Sulfate

Monday, May 9th, 2011

Background:

Fluorescence spectroscopy analysis is a great tool for investigational research and analytical science applications. It is used often in biochemical, chemical, pharmaceutical, and medical applications, in addition to mineralogy, fluorescent labeling, sensors and forensics applications. It is also used to aid in the identification of proteins, organic compounds, oils and dyes and is used for environmental monitoring and laser induced chlorophyll fluorescence for crop yield assessments. (more…)

Fluorescence of Detergent Samples Using a Reflection Probe

Wednesday, March 10th, 2010

Goal:
Determine the feasibility of using the Ocean Optics reflection probes to measure the fluorescence of detergent samples. (more…)

Effect of fiber diameter on fluorescein fluorescence

Monday, October 26th, 2009

Goal:
Assess the effect of various fiber diameters on fluorescein fluorescence (more…)

Analysis of FITC and Cy5 Fluorescent Probes

Thursday, October 15th, 2009

Goal:
To determine the optimal system configuration and optical filters for detecting fluorescent probes (FITC and Cy5) incorporated into DNA during PCR using proprietary technology and primers (more…)

Analysis of FRET Samples

Thursday, October 15th, 2009

Goal:
To assess feasibility and determine the optimal system configuration for detecting Cy5 fluorescence in samples labeled for fluorescence energy transfer from fluorescein to Cy5 (more…)