Goal:
Assess the effect of various fiber diameters on fluorescein fluorescence

Method:
Fluorescence

Hardware Used:
USB2000-FL (USB2E3295)
USB-LS-450 (U45EA229)
CUV-ALL-UV
P400, P600, P1000 UV/VIS optical fibers

Acquisition Parameters:
Integration Time: 100 msec
Spectra Averaged: 1
Boxcar Smoothing: 10

Measurement Mode:
Fluorescence

Experimental Conditions:
10 uM fluorescein contained in a disposable cuvette (CVD-UV1S) (cuvette oriented with 1 cm pathlength towards spectrometer)

Results:
Fluorescein fluorescence measured with various combinations of 400 to 1000 micron optical fibers is shown in the figure below.

Conclusions:
For fluorescence measurements with the CUV-ALL-UV (90 degree configuration), the optimal optical fiber sizes for detecting fluorescein fluorescence are a 1000 micron fiber on the illumination/excitation side (from light source to cuvette holder) and 1000 micron fiber on the read/detection side (from cuvette holder to spectrometer). As shown in the figure, the use of a 600 micron fiber on the detection side does not have a significant impact on the fluorescence intensity measured.

These results can most likely be extrapolated for other fluorophores meaning ideal fiber sizes for fluorescence measurements are 1000 um fibers.

Tags: fiber, Fluorescence, fluroescein

This entry was posted on Monday, October 26th, 2009 at 9:56 am and is filed under Chemistry, Fluorescence. You can follow any responses to this entry through the RSS 2.0 feed. Both comments and pings are currently closed.